Atlantic Blue™ Anti-Mouse PD-1/CD279 (RMP1-30)

PD-1/CD279 Monoclonal Antibody for FC
Cat No. AB-65142

产品说明书

CloneNo. RMP1-30

宿主/亚型

Rat / IgG2b, kappa

种属反应性

Mouse

应用

FC

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC detected inAnti-CD3 and Anti-CD28 stimulated mouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

AB-65142 targets PD-1/CD279 in FC applications and shows reactivity with Mouse samples.

经测试应用 FC Application Description
经测试反应性 Mouse
免疫原 Mouse PD-1 transfected BHK cells 种属同源性预测
宿主/亚型 Rat / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1
GenBank蛋白编号BC119179
基因名称 PD-1
Gene ID (NCBI) 18566
RRIDAB_2935176
偶联类型 Atlantic Blue™ Fluorescent Dye
最大激发/发射波长404 nm / 458 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02242
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for Atlantic Blue™ PD-1/CD279 antibody AB-65142Download protocol
Standard Protocols
Click here to view our Standard Protocols
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