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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    FC experiment of human PBMCs using 98032-1-RR (same clone as 98032-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98032-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98032-1-RR

    Affinity and Kinetic Characterization of 98032-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98032-1-RR against Human CD28 were performed. The affinity constant is 3.23 nM.

Anti-Human CD28 Rabbit Recombinant Antibody, PBS Only

CD28 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98032-1-PBS

Print datasheet

CloneNo. 240354D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

98032-1-PBS targets CD28 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD28 molecule
Synonyms Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2
Calculated Molecular Weight 220 aa, 25 kDa
GenBank Accession NumberBC093698
Gene Symbol CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10747
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).