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Anti-Human CD28 Rabbit Recombinant Antibody

CD28 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240354D2

Cat No : 98032-1-RR

Print datasheet

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

验证数据展示

  • FC experiment of human PBMCs using 98032-1-RR

    FC experiment of human PBMCs using 98032-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98032-1-RR

    FC experiment of human PBMCs using 98032-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR, Clone: 240354D2) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity experiments of 98032-1-RR

    Affinity experiments of 98032-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98032-1-RR against Human CD28 were performed. The affinity constant is 3.23 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98032-1-RR is CD28 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD28 molecule
Synonyms Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2
Calculated Molecular Weight 220 aa, 25 kDa
GenBank Accession NumberBC093698
Gene Symbol CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10747
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

实验方案

Product Specific Protocols
FC protocol for CD28 antibody 98032-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols