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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65601-1-MR

    FC experiment of human PBMCs using 65601-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD45. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 65601-1-MR

    FC experiment of human PBMCs using 65601-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (65601-1-MR, Clone: TS2/16) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant Antibody

CD29 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65601-1-MR

Print datasheet

CloneNo. TS2/16

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65601-1-MR targets CD29 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Synonyms ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA
Calculated Molecular Weight 88 kDa
GenBank Accession NumberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
RRIDAB_3670348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

实验方案

Product Specific Protocols
FC protocol for CD29 antibody 65601-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols