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CoraLite® Plus 488 Anti-Human CD29 (TS2/16) Mouse IgG2a Recombinant Antibody

CD29 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

TS2/16

Cat no : CL488-65601

Print datasheet

Synonyms

ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA

验证数据展示

  • FC experiment of human PBMCs using CL488-65601

    FC experiment of human PBMCs using CL488-65601

    1x10^6 human PBMCs were surface stained with APC Anti-Human CD45 and CoraLite® Plus 488 Anti-Human CD29 (TS2/16) (CL488-65191, Clone: TS2/16) (right), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (left). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-65601

    FC experiment of human PBMCs using CL488-65601

    1x10^6 human PBMCs were surface stained with 5ul CoraLite® Plus 488 Anti-Human CD29 (TS2/16) (CL488-65191, Clone: TS2/16) (red), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65601-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65601 targets CD29 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Synonyms ITGB1, ITB1, Integrin beta-1, Integrin beta 1, GPIIA
Calculated Molecular Weight 88 kDa
GenBank Accession NumberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD29 antibody CL488-65601Download protocol
Standard Protocols
Click here to view our Standard Protocols