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验证数据展示

  • FC experiment of human PBMCs using 98188-1-RR

    FC experiment of human PBMCs using 98188-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 Rabbit Recombinant Antibody (98188-1-RR, Clone: 241685C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98188-1-RR

    FC experiment of human PBMCs using 98188-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD29 Rabbit Recombinant Antibody (98188-1-RR, Clone: 241685C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98188-1-RR

    Affinity and Kinetic Characterization of 98188-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98188-1-RR against Human CD29 were performed. The affinity constant is 0.657 nM.

Anti-Human CD29 Rabbit Recombinant Antibody

CD29 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98188-1-RR

产品说明书

CloneNo. 241685C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

ITGB1, Integrin beta-1, Integrin beta 1, GPIIA, Glycoprotein IIa

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98188-1-RR targets CD29 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
别名 ITGB1, Integrin beta-1, Integrin beta 1, GPIIA, Glycoprotein IIa
计算分子量 88 kDa
GenBank蛋白编号BC020057
基因名称 Integrin beta 1
Gene ID (NCBI) 3688
ENSEMBL Gene IDENSG00000150093
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP05556
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

实验方案

Product Specific Protocols
FC protocol for CD29 antibody 98188-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols