验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy ITGB1/CD29 Ready-To-Use IHC Kit. ITGB1/CD29 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65191-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-65191 targets CD29 in FC applications and shows reactivity with Human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | Human |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) |
| 别名 | CD29, FNRB, GPIIA, Integrin beta 1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB |
| 计算分子量 | 88 kDa |
| GenBank蛋白编号 | BC020057 |
| 基因名称 | Integrin beta 1 |
| Gene ID (NCBI) | 3688 |
| ENSEMBL Gene ID | ENSG00000150093 |
| RRID | AB_2934658 |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05556 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 CD29 antibody CL555-65191 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |