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验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG Isotype Control, and FITC anti-Armenian Hamster IgG antibody at dilution 1:100. Cells were not fixed.

  • IF Staining of mouse splenocytes using 65060-1-Ig

    IF Staining of mouse splenocytes using 65060-1-Ig

    Immunofluorescent analysis of mouse splenocytes using CD3ε antibody (65060-1-Ig, Clone: 145-2C11 ) at dilution of 1:500 and FITC Goat anti-Hamster(Armenian) IgG.

Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for IF/ICC, FC
Cat No. 65060-1-Ig

产品说明书

CloneNo. 145-2C11

宿主/亚型

Hamster / IgG

种属反应性

mouse

应用

IF/ICC, FC

别名

Cd3e, T3e, CD3epsilon, CD3, AI504783

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inC57BL/6 mouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65060-1-Ig targets CD3ε in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 种属同源性预测
宿主/亚型 Hamster / IgG
抗体类别 Monoclonal
产品类型 Antibody
全称 CD3 antigen, epsilon polypeptide
别名 Cd3e, T3e, CD3epsilon, CD3, AI504783
GenBank蛋白编号BC098236
基因名称 CD3ε
Gene ID (NCBI) 12501
RRIDAB_2918368
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP22646
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
IF protocol for CD3ε antibody 65060-1-IgDownload protocol
FC protocol for CD3ε antibody 65060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols