• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

145-2C11

Cat no : CL647-65060

Print datasheet

Synonyms

Cd3e, T3e, CD3epsilon, CD3, AI504783

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65060

    FC experiment of mouse splenocytes using CL647-65060

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone:145-2C11) or 0.25 ug Isotype Control, and 0.25 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65060-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65060 targets CD3ε in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 种属同源性预测
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Full Name CD3 antigen, epsilon polypeptide
Synonyms Cd3e, T3e, CD3epsilon, CD3, AI504783
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD3ε antibody CL647-65060Download protocol
Standard Protocols
Click here to view our Standard Protocols