• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD3 (Hit3a)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

Hit3a

Cat no : 65112-1-Ig

Print datasheet

Synonyms

CD3, CD3E, T3E, TCRE

验证数据展示

  • FC experiment of human PBMCs using 65112-1-Ig

    FC experiment of human PBMCs using 65112-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL750 Anti-Human CD45. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65112-1-Ig

    FC experiment of human PBMCs using 65112-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD3 (65112-1-Ig, Clone:Hit3a) or Mouse IgG2a Isotype Control and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65112-1-Ig targets CD3 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3, CD3E, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_2918410
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP07766
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for CD3 antibody 65112-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols