APC Anti-Human CD3 (Hit3a)
CD3 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
Hit3a
验证数据展示
经过测试的应用
| Positive FC detected in | Human peripheral blood lymphocytes |
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-65112 targets CD3 in FC applications and shows reactivity with Human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Human |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Synonyms | CD3, CD3E, T3E, TCRE |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| RRID | AB_2883001 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P07766 |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD3 antibody APC-65112 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
