验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells, pig thymus tissue, rat thymus tissue, mouse thymus tissue |
| Positive IHC detected in | human colon tissue, human tonsillitis tissue, mouse small intestine tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, mouse small intestine tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81324-1-RR targets CD3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | CD3 fusion protein Ag11797 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Synonyms | CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| Observed Molecular Weight | 23-25 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| RRID | AB_2935548 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD3 antibody 81324-1-RR | Download protocol |
| IHC protocol for CD3 antibody 81324-1-RR | Download protocol |
| IF protocol for CD3 antibody 81324-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
