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- KD/KO Validated
CoraLite® Plus 488-conjugated CD3 Recombinant antibody
CD3 Uni-rAbTM Recombinant Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4I19
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse spleen tissue |
| Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81324-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81324 targets CD3 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | CD3 fusion protein Ag11797 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Synonyms | |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| Observed Molecular Weight | 23-25 kDa |
| GenBank Accession Number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07766 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a monoclonal antibody directed against the epsilon chain of human CD3 molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD3 antibody CL488-81324 | Download protocol |
| FC protocol for CL Plus 488 CD3 antibody CL488-81324 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |