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Biotin Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody

CD3 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Biotin

CloneNo.

OKT3

Cat No : Biotin-65569

Print datasheet

Synonyms

CD3E, T3E, TCRE

验证数据展示

  • FC experiment of human PBMCs using Biotin-65569

    FC experiment of human PBMCs using Biotin-65569

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) or 0.25 ug Isotype Control, and CoraLite®488-conjugated streptavidin. Cells were then stained with CoraLite® Plus 405 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-65569

    FC experiment of human PBMCs using Biotin-65569

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin CD3 Recombinant Antibody (Biotin-65569, Clone: OKT3) and CoraLite®488-conjugated streptavidin (red) or 0.25 ug Isotype Control and CoraLite®488-conjugated streptavidin (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65569 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3E, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3E
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for Biotin CD3 antibody Biotin-65569Download protocol
Standard Protocols
Click here to view our Standard Protocols