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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CD3 Polyclonal antibody

CD3 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-17617

Print datasheet

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

验证数据展示

  • IF Staining of mouse thymus using CL647-17617

    IF Staining of mouse thymus using CL647-17617

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CoraLite® Plus 647 CD3 antibody (CL647-17617) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse thymus tissue
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17617-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-17617 targets CD3 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CD3 fusion protein Ag11797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07766
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD3 antibody CL647-17617Download protocol
Standard Protocols
Click here to view our Standard Protocols