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FITC Plus Anti-Human CD3 (UCHT1) Mouse IgG1 Recombinant Antibody

CD3 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

UCHT1

Cat No : FITC-65536

Print datasheet

Synonyms

CD3E, T3E, TCRE

验证数据展示

  • FC experiment of human PBMCs using FITC-65536

    FC experiment of human PBMCs using FITC-65536

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 Mouse Recombinant Antibody (FITC-65536, Clone: UCHT1) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21), and APC Anti-Human CD19 (HIB19) (APC-65110, Clone: HIB19). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65536

    FC experiment of human PBMCs using FITC-65536

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65536, Clone:UCHT1)(red), and 5 ul FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21)(blue). Cells were not fixed. Overlap OEM FITC-65151 21009051(green)

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65536 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3E, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3E
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD3 antibody FITC-65536Download protocol
Standard Protocols
Click here to view our Standard Protocols