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验证数据展示

  • WB analysis using 84836-2-RR

    WB analysis using 84836-2-RR

    Various cells were subjected to SDS PAGE followed by western blot with 84836-2-RR (CD3 Gamma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84836-2-RR

    IHC staining of human tonsillitis using 84836-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84836-2-RR (CD3 Gamma antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84836-2-RR

    IHC staining of human tonsillitis using 84836-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84836-2-RR (CD3 Gamma antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD3 Gamma Recombinant antibody

CD3 Gamma Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84836-2-RR

产品说明书

CloneNo. 241849D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD3G, 241849D4, T-cell receptor T3 gamma chain, T3G, T cell receptor T3 gamma chain

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84836-2-RR targets CD3 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CD3 Gamma fusion protein Eg2147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD3g molecule, gamma (CD3-TCR complex)
别名 CD3G, 241849D4, T-cell receptor T3 gamma chain, T3G, T cell receptor T3 gamma chain
计算分子量 182 aa, 20 kDa
观测分子量22 kDa
GenBank蛋白编号BC113830
基因名称 CD3 Gamma
Gene ID (NCBI) 917
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP09693
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

实验方案

Product Specific Protocols
WB protocol for CD3 Gamma antibody 84836-2-RRDownload protocol
IHC protocol for CD3 Gamma antibody 84836-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols