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CD31 Recombinant antibody, PBS Only

CD31 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4N13

Cat no : 80530-1-PBS

Print datasheet

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

验证数据展示

  • WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

    IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (80530-1-RR, Clone: 4N13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80530-1-RR

    Affinity and Kinetic Characterization of 80530-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80530-1-RR against Human CD31 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

产品信息

80530-1-PBS targets CD31 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD31 fusion protein Ag1787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16284
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).