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验证数据展示

  • WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

    IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (80530-1-RR, Clone: 4N13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80530-1-RR (same clone as 80530-1-PBS)

    IHC staining of human tonsillitis using 80530-1-RR (same clone as 80530-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80530-1-RR (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD31 antibody included
  • Affinity and Kinetic Characterization of 80530-1-RR

    Affinity and Kinetic Characterization of 80530-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80530-1-RR against Human CD31 were performed. The affinity constant is below 1 pM.

CD31 Recombinant antibody, PBS Only

CD31 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80530-1-PBS

产品说明书

CloneNo. 4N13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

产品信息

80530-1-PBS targets CD31 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CD31 fusion protein Ag1787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
计算分子量 83 kDa
观测分子量 130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2918900
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16284
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).