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CD31 Recombinant antibody

CD31 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4N13

Cat no : 80530-1-RR

Print datasheet

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

验证数据展示

  • WB analysis using 80530-1-RR

    WB analysis using 80530-1-RR

    Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80530-1-RR

    WB analysis using 80530-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of bEnd.3 using 80530-1-RR

    IF Staining of bEnd.3 using 80530-1-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (80530-1-RR, Clone: 4N13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 80530-1-RR

    Affinity and Kinetic Characterization of 80530-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80530-1-RR against Human CD31 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, MOLT-4 cells, Jurkat cells
Positive IF/ICC detected inbEnd.3 cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

80530-1-RR targets CD31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen CD31 fusion protein Ag1787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_2918900
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
WB protocol for CD31 antibody 80530-1-RRDownload protocol
IF protocol for CD31 antibody 80530-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Ecotoxicol Environ Saf

Polystyrene nanoplastics accelerate atherosclerosis: Unraveling the impact on smooth muscle cells through KIF15-mediated migration

Authors - Yizhou Zhong
View Article