• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HUVEC using CL488-80530

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HUVEC using CL488-80530

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

CoraLite® Plus 488-conjugated CD31 Recombinant antibody

CD31 Recombinant Antibody for IF/ICC
Cat No. CL488-80530

产品说明书

CloneNo. 4N13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80530-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80530 targets CD31 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 CD31 fusion protein Ag1787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
计算分子量 83 kDa
观测分子量 130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_3673033
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16284
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-80530Download protocol
Standard Protocols
Click here to view our Standard Protocols