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CoraLite® Plus 488-conjugated CD31 Recombinant antibody

CD31 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4N13

Cat no : CL488-80530

Print datasheet

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

验证数据展示

  • IF Staining of HUVEC using CL488-80530

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HUVEC using CL488-80530

    IF Staining of HUVEC using CL488-80530

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-80530, Clone: 4N13 ) at dilution of 1:100, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80530-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80530 targets CD31 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen CD31 fusion protein Ag1787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16284
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-80530Download protocol
Standard Protocols
Click here to view our Standard Protocols