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Anti-Mouse CD31 Rabbit Recombinant Antibody

CD31 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

242041B5

Cat no : 98258-1-RR

Print datasheet

Synonyms

PECAM-1, Pecam1, PECAM 1, Pecam, 242041B5

验证数据展示

  • FC experiment of mouse splenocytes using 98258-1-RR

    FC experiment of mouse splenocytes using 98258-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit Recombinant Antibody (98258-1-RR, Clone: 242041B5) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

  • FC experiment of mouse splenocytes using 98258-1-RR

    FC experiment of mouse splenocytes using 98258-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit Recombinant Antibody (98258-1-RR, Clone:242041B5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98258-1-RR

    Affinity and Kinetic Characterization of 98258-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98258-1-RR against Mouse CD31 were performed. The affinity constant is 0.547 nM.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98258-1-RR targets CD31 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name platelet/endothelial cell adhesion molecule 1
Synonyms PECAM-1, Pecam1, PECAM 1, Pecam, 242041B5
Calculated Molecular Weight81 kDa
GenBank Accession NumberNM_001305157.1
Gene Symbol CD31
Gene ID (NCBI) 18613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08481-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
FC protocol for CD31 antibody 98258-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols