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验证数据展示

  • FC experiment of human PBMCs using FITC-65614

    FC experiment of human PBMCs using FITC-65614

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (FITC-65614, Clone: WM-59) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using FITC-65614

    FC experiment of human PBMCs using FITC-65614

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (FITC-65614, Clone: WM-59) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed.

FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant Antibody

CD31 Uni-rAbTM Recombinant Antibody for FC
Cat No. FITC-65614

产品说明书

CloneNo. WM-59

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC Plus
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65614 targets CD31 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 PECAM1, PECAM 1, PECA1, GPIIA', GPIIA
计算分子量 83 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD31 antibody FITC-65614Download protocol
Standard Protocols
Click here to view our Standard Protocols