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验证数据展示

  • FC experiment of human PBMCs using FITC-65614

    FC experiment of human PBMCs using FITC-65614

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (FITC-65614, Clone: WM-59) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 (26IC) (CL647-65246, Clone: 26IC). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using FITC-65614

    FC experiment of human PBMCs using FITC-65614

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a RecAb (FITC-65614, Clone: WM-59) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed.

FITC Plus Anti-Human CD31 (WM-59) Mouse IgG2a Recombinant Antibody

CD31 Uni-rAbTM Recombinant Antibody for FC
Cat No. FITC-65614

Print datasheet

CloneNo. WM-59

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65614 targets CD31 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM1, PECAM 1, PECA1, GPIIA', GPIIA
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD31 antibody FITC-65614Download protocol
Standard Protocols
Click here to view our Standard Protocols