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Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

1D11

Cat no : 65188-1-Ig

Print datasheet

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

验证数据展示

  • FC experiment of human PBMCs using 65188-1-Ig

    FC experiment of human PBMCs using 65188-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were then stained with CL488-conjugated CD8 (CL488-65135). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65188-1-Ig

    FC experiment of human PBMCs using 65188-1-Ig

    1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD314/NKG2D (65188-1-Ig, Clone:1D11) or Mouse IgG1 Isotype Control and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65188-1-Ig targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen NKL cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily K, member 1
Synonyms CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC039836
Gene Symbol KLRK1
Gene ID (NCBI) 22914
RRIDAB_2918459
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP26718
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

实验方案

Product Specific Protocols
FC protocol for CD314/NKG2D antibody 65188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols