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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL594-65188

    FC experiment of human PBMCs using CL594-65188

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD8 and 5 ul CoraLite® Plus 594 Anti-Human CD314/NKG2D (CL594-65188, Clone:1D11) or CoraLite® Plus 594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL594-65188

    FC experiment of human PBMCs using CL594-65188

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD314/NKG2D (CL594-65188, Clone:1D11) or CoraLite® Plus 594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 594 Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC
Cat No. CL594-65188

产品说明书

CloneNo. 1D11

宿主/亚型

Mouse / IgG1, kappa

种属反应性

Human

应用

FC

别名

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 594
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65188-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65188 targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 NKL cell line 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 killer cell lectin-like receptor subfamily K, member 1
别名 CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D
计算分子量 25 kDa
GenBank蛋白编号BC039836
基因名称 KLRK1
Gene ID (NCBI) 22914
RRIDAB_3673459
偶联类型 CoraLite® Plus 594 Fluorescent Dye
最大激发/发射波长594 nm / 615 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP26718
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD314/NKG2D antibody CL594-65188Download protocol
Standard Protocols
Click here to view our Standard Protocols