CoraLite® Plus 488 Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody

CD324 (E-cadherin) Uni-rAbTM Recombinant Antibody for FC
Cat No. CL488-98123

产品说明书

CloneNo. 241098F7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98123-1-PBS

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98123 targets CD324 (E-cadherin) in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1
计算分子量97 kDa
GenBank蛋白编号NM_004360.5
基因名称 E-cadherin
Gene ID (NCBI) 999
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP12830-1
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD324 (E-cadherin) antibody CL488-98123Download protocol
Standard Protocols
Click here to view our Standard Protocols
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