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CD34 Recombinant antibody, PBS Only

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242069F8

Cat no : 84242-5-PBS

Print datasheet

Synonyms

CD34 molecule, 242069F8

验证数据展示

  • WB analysis using 84242-5-RR (same clone as 84242-5-PBS)

    WB analysis using 84242-5-RR (same clone as 84242-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    IHC staining of human placenta using 84242-5-RR (same clone as 84242-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84242-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD34 antibody included
  • Affinity and Kinetic Characterization of 84242-5-RR

    Affinity and Kinetic Characterization of 84242-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.

产品信息

84242-5-PBS targets CD34 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD34 molecule
Synonyms CD34 molecule, 242069F8
Calculated Molecular Weight 41 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP28906
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.