• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84242-5-RR

    WB analysis using 84242-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84242-5-RR (CD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84242-5-RR

    IHC staining of human placenta using 84242-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human placenta using 84242-5-RR

    IHC staining of human placenta using 84242-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84242-5-RR (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • Affinity and Kinetic Characterization of 84242-5-RR

    Affinity and Kinetic Characterization of 84242-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84242-5-RR against Human CD34 were performed. The affinity constant is 16.3 nM.

CD34 Recombinant antibody

CD34 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84242-5-RR

产品说明书

CloneNo. 242069F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

242069F8, CD34 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHL-60 cells, bEnd.3 cells, HUVEC cells, mouse testis tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue, human colon cancer tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84242-5-RR targets CD34 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD34 molecule
别名 242069F8, CD34 molecule
计算分子量 41 kDa
观测分子量105 kDa
GenBank蛋白编号BC039146
基因名称 CD34
Gene ID (NCBI) 947
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP28906
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
WB protocol for CD34 antibody 84242-5-RRDownload protocol
IHC protocol for CD34 antibody 84242-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols