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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL647-65254

    FC experiment of human PBMCs using CL647-65254

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD35 (CL647-65254, Clone: E11), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65254

    FC experiment of human PBMCs using CL647-65254

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD35 (CL647-65254, Clone: E11), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 647 Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for FC
Cat No. CL647-65254

产品说明书

CloneNo. E11

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

KN, E11, CR1, Complement receptor type 1, C3BR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65254-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65254 targets CD35 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3b/4b) receptor 1 (Knops blood group)
别名 KN, E11, CR1, Complement receptor type 1, C3BR
计算分子量 224 kDa
GenBank蛋白编号NM_000573
基因名称 CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_2935033
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP17927
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD35 antibody CL647-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols