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Anti-Human CD36 Rabbit Recombinant Antibody

CD36 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241262D5

Cat no : 98132-1-RR

Print datasheet

Synonyms

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

验证数据展示

  • FC experiment of human PBMCs using 98132-1-RR

    FC experiment of human PBMCs using 98132-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD36 Rabbit Recombinant Antibody (98132-1-RR, Clone: 241262D5) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL647-65560, Clone: L243). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98132-1-RR

    FC experiment of human PBMCs using 98132-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD36 Rabbit Recombinant Antibody (98132-1-RR, Clone: 241262D5) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98132-1-RR

    Affinity and Kinetic Characterization of 98132-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98132-1-RR against Human CD36 were performed. The affinity constant is 42.1 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98132-1-RR targets CD36 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD36 molecule (thrombospondin receptor)
Synonyms GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb
Calculated Molecular Weight53 kDa
GenBank Accession NumberNM_001001548.3
Gene Symbol CD36
Gene ID (NCBI) 948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16671-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.

实验方案

Product Specific Protocols
FC protocol for CD36 antibody 98132-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols