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验证数据展示

  • FC experiment of human peripheral blood platelets using CL647-65248

    FC experiment of human peripheral blood platelets using CL647-65248

    1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD36 (CL647-65248, Clone:SMΦ), or CoraLite® Plus 647 Mouse IgM Isotype Control. Cells were not fixed. CD41a positive cells were gated.

  • FC experiment of human peripheral blood platelets using CL647-65248

    FC experiment of human peripheral blood platelets using CL647-65248

    1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD36 (CL647-65248, Clone:SMΦ), or CoraLite® Plus 647 Mouse IgM Isotype Control. Cells were not fixed. CD41a positive cells were gated.

CoraLite® Plus 647 Anti-Human CD36 (SMΦ)

CD36 Monoclonal Antibody for FC
Cat No. CL647-65248

产品说明书

CloneNo. SMΦ

宿主/亚型

Mouse / IgM, kappa

种属反应性

Human

应用

FC

别名

CD36, CHDS7, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, PAS 4, PAS IV, PASIV, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, SCARB3, Thrombospondin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 65248-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65248 targets CD36 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgM, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD36 molecule (thrombospondin receptor)
别名 CD36, CHDS7, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, PAS 4, PAS IV, PASIV, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, SCARB3, Thrombospondin receptor
计算分子量 472 aa, 53 kDa
GenBank蛋白编号BC008406
基因名称 CD36
Gene ID (NCBI) 948
RRIDAB_3084811
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP16671
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD36, also named as GP3B and GP4, is an 88-kDa membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutation of CD36 will cause platelet glycoprotein IV deficiency which known as CD36 deficiency. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD36 antibody CL647-65248Download protocol
Standard Protocols
Click here to view our Standard Protocols