PE Anti-Rat CD4 (OX-35)
CD4 Monoclonal Antibody for FC
Host / Isotype
Mouse / Mouse IgG2a kappa
Reactivity
Rat
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
OX-35
验证数据展示
经过测试的应用
| Positive FC detected in | rat splenocytes |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-65179 targets CD4 in FC applications and shows reactivity with Rat samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Rat |
| Immunogen | MLR generated rat T helper lymphocytes 种属同源性预测 |
| Host / Isotype | Mouse / Mouse IgG2a kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Cd4 molecule |
| Synonyms | CD4, CD4 molecule, p55, W3/25 |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 24932 |
| RRID | AB_2883951 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Storage Buffer | PBS with 0.1% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD4 antibody PE-65179 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
