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PE-Cyanine7 Anti-Human CD4 (SK3)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

PE-Cyanine7 Fluorescent Dye

CloneNo.

SK3

Cat no : PY7-65147

Print datasheet

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, SK3, CD4mut, CD4 molecule

验证数据展示

  • FC experiment of human PBMCs using PY7-65147

    FC experiment of human PBMCs using PY7-65147

    1x10^6 human PBMCs were surface stained with 5 ul PE-Cyanine7 Anti-Human CD4 (SK3) (PY7-65147, Clone: SK3) or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PY7-65147 targets CD4 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, SK3, CD4mut, CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate PE-Cyanine7 Fluorescent Dye
Excitation/Emission Maxima Wavelengths450-500 nm / 778 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for PE-Cyanine7 CD4 antibody PY7-65147Download protocol
Standard Protocols
Click here to view our Standard Protocols