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Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody, PBS Only

CD40L/CD154 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240743E6

Cat no : 98120-1-PBS

Print datasheet

Synonyms

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand

验证数据展示

  • FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

    FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

    1x10^6 untreated or PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98120-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

    FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

    1x10^6 PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98120-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98120-1-RR

    Affinity and Kinetic Characterization of 98120-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98120-1-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.989 nM.

产品信息

98120-1-PBS targets CD40L/CD154 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD40 ligand
Synonyms CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand
Calculated Molecular Weight29 kDa
GenBank Accession NumberNM_011616.2
Gene Symbol CD154
Gene ID (NCBI) 21947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27548
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.