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Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AK2

Cat no : 65163-1-Ig

Print datasheet

Synonyms

AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα

验证数据展示

  • FC experiment of human peripheral blood platelets using 65163-1-Ig

    FC experiment of human peripheral blood platelets using 65163-1-Ig

    1X10^6 human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CoraLite® Plus488 Antibody Labeling Kit for Mouse IgG1 (KFA021) and 0.2 ug Anti-Human CD42b (65163-1-Ig, Clone:AK2) labeled with FlexAble CoraLite® PlusCL647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or unstained. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood platelets

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65163-1-Ig targets CD42b in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human platelets 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycoprotein Ib (platelet), alpha polypeptide
Synonyms AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα
Calculated Molecular Weight 626 aa, 69 kDa
GenBank Accession NumberBC027955
Gene Symbol CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_2881480
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP07359
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

实验方案

Product Specific Protocols
FC protocol for CD42b antibody 65163-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols