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验证数据展示

  • FC experiment of human peripheral blood platelets using CL405-65163

    FC experiment of human peripheral blood platelets using CL405-65163

    1X10^6 human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) and 5 ul CoraLite® Plus 405 Anti-Human CD42b (CL405-65163, Clone:AK2) or Mouse IgG1 Isotype Control. Cells were not fixed.

  • FC experiment of human peripheral blood platelets using CL405-65163

    FC experiment of human peripheral blood platelets using CL405-65163

    1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD42b (CL405-65163, Clone:AK2) or Mouse IgG1 Isotype Control. Cells were not fixed.

CoraLite® Plus 405 Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC
Cat No. CL405-65163

产品说明书

CloneNo. AK2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

FC

别名

BSS, CD42B, CD42b alpha, GP1B, GP1BA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 405
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 65163-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65163 targets CD42b in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Human platelets 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycoprotein Ib (platelet), alpha polypeptide
别名 BSS, CD42B, CD42b alpha, GP1B, GP1BA
计算分子量 626 aa, 69 kDa
GenBank蛋白编号BC027955
基因名称 CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_3083836
偶联类型 CoraLite® Plus 405 Fluorescent Dye
最大激发/发射波长399 nm / 422 nm
形式 Liquid
纯化方式Purified by protein-A affinity chromatography
UNIPROT IDP07359
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD42b antibody CL405-65163Download protocol
Standard Protocols
Click here to view our Standard Protocols