- Featured Product
- KD/KO Validated
CD44 Polyclonal antibody
CD44 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)
Conjugate
Unconjugated
332
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HUVEC cells, MDA-MB-231 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
Positive IF/ICC detected in | HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15675-1-AP targets CD44 in WB, IHC, IF/ICC, IF-P, IP, CoIP, Neutralization, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, Neutralization |
Tested Reactivity | human |
Cited Reactivity | human, bovine, sika deer |
Immunogen | CD44 fusion protein Ag8143 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD44 molecule (Indian blood group) |
Synonyms | Epican, ECMR-III, ECMR III, CSPG8, CDW44 |
Calculated Molecular Weight | 742 aa, 82 kDa |
Observed Molecular Weight | 80-95 kDa |
GenBank Accession Number | BC004372 |
Gene Symbol | CD44 |
Gene ID (NCBI) | 960 |
ENSEMBL Gene ID | ENSG00000026508 |
RRID | AB_2076198 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P16070 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD44 antibody 15675-1-AP | Download protocol |
IHC protocol for CD44 antibody 15675-1-AP | Download protocol |
IF protocol for CD44 antibody 15675-1-AP | Download protocol |
IP protocol for CD44 antibody 15675-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nanomicro Lett Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling | ||
J Clin Invest LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis | ||
Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss | ||
Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma |