验证数据展示

WB analysis of HeLa cells using 15675-1-AP
WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.
WB analysis using 15675-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 15675-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human lung cancer using 15675-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD44 antibody included
IHC staining of human lung cancer using 15675-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD44 antibody included
IP experiment of HeLa using 15675-1-AP
IP result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.
IF Staining of human colon cancer using 15675-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (15675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human colon cancer using 15675-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (15675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 15675-1-AP

产品说明书

引用文献(350)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (3)

别名

CD44 antigen, CDW44, CSPG8, ECMR III, ECMR-III

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (350)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A549 cells, HeLa cells, HUVEC cells, MDA-MB-231 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human colon cancer tissue
Positive IF/ICC detected in	HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells

Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 14 publications below
WB	See 240 publications below
IHC	See 89 publications below
IF	See 89 publications below
IP	See 1 publications below
FC	See 9 publications below
CoIP	See 3 publications below

产品信息

15675-1-AP targets CD44 in WB, IHC, IF/ICC, IF-P, IP, CoIP, Neutralization, ELISA applications and shows reactivity with human samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, Neutralization
经测试反应性	human
文献引用反应性	human, bovine, sika deer
免疫原	CD44 fusion protein Ag8143 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	CD44 molecule (Indian blood group)
别名	CD44 antigen, CDW44, CSPG8, ECMR III, ECMR-III
计算分子量	742 aa, 82 kDa
观测分子量	80-95 kDa
GenBank蛋白编号	BC004372
基因名称	CD44
Gene ID (NCBI)	960
ENSEMBL Gene ID	ENSG00000026508
RRID	AB_2076198
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P16070
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 15675-1-AP	Download protocol
IHC protocol for CD44 antibody 15675-1-AP	Download protocol
IF protocol for CD44 antibody 15675-1-AP	Download protocol
IP protocol for CD44 antibody 15675-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	IF	Nature ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis. Authors - Ting Zhang View Article
	IF	Nanomicro Lett Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling Authors - Junji Ren View Article
mouse	IF	Nat Commun Hyperthermia-triggered biomimetic bubble nanomachines Authors - Junbin Gao View Article
human	WB	J Clin Invest LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis Authors - Yingzhi Shen View Article
mouse	IF	Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss Authors - Yuxiang Hu View Article
human	WB,IHC,IF	Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma Authors - Yuantong Liu View Article

验证数据展示

WB analysis of HeLa cells using 15675-1-AP

WB analysis using 15675-1-AP

IF Staining of HeLa using 15675-1-AP

IHC staining of human lung cancer using 15675-1-AP

IHC staining of human lung cancer using 15675-1-AP

IP experiment of HeLa using 15675-1-AP

IF Staining of human colon cancer using 15675-1-AP

IF Staining of human colon cancer using 15675-1-AP

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.

Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling

Hyperthermia-triggered biomimetic bubble nanomachines

LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis

The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma