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验证数据展示

  • IF Staining of HeLa using CL488-15675

    IF Staining of HeLa using CL488-15675

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CD44 antibody (CL488-15675) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD44 Polyclonal antibody

CD44 Polyclonal Antibody for IF/ICC
Cat No. CL488-15675

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15675-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15675 targets CD44 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CD44 fusion protein Ag8143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD44 molecule (Indian blood group)
Synonyms Epican, ECMR-III, ECMR III, CSPG8, CDW44
Calculated Molecular Weight 742 aa, 82 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_2919121
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16070
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD44 antibody CL488-15675Download protocol
Standard Protocols
Click here to view our Standard Protocols