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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65502-1-PBS

    FC experiment of human PBMCs using 65502-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-PBS, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65502-1-RR (same clone as 65502-1-PBS)

    FC experiment of human PBMCs using 65502-1-RR (same clone as 65502-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Titrations: 0.4, 0.2, 0.1, 0.05ug This data was developed using the same antibody clone with 65502-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65502-1-RR (same clone as 65502-1-PBS)

    FC experiment of human PBMCs using 65502-1-RR (same clone as 65502-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-RR, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65502-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65502-1-PBS

    FC experiment of human PBMCs using 65502-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD44 Rabbit Recombinant Antibody (65502-1-PBS, Clone:F10-44-2) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD44 (F10-44-2) Rabbit IgG Recombinant Antibody, PBS Only

CD44 Recombinant Antibody for FC
Cat No. 65502-1-PBS

Print datasheet

CloneNo. F10-44-2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65502-1-PBS targets CD44 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Purified T cells from human lymph nodes 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD44 molecule (Indian blood group)
Synonyms Epican, ECMR-III, ECMR III, CSPG8, CDW44
Calculated Molecular Weight 742 aa, 82 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
FC protocol for CD44 antibody 65502-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols