• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using CL488-65063

    FC experiment of human PBMCs using CL488-65063

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD44 (CL488-65063, Clone:F10-44-2) (red) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL488-65063

    FC experiment of human PBMCs using CL488-65063

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD44 (CL488-65063, Clone:F10-44-2) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 488 Anti-Human CD44 (F10-44-2)

CD44 Monoclonal Antibody for FC
Cat No. CL488-65063

产品说明书

CloneNo. F10-44-2

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

Human

应用

FC

别名

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65063-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65063 targets CD44 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Purified T cells from human lymph nodes 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD44 molecule (Indian blood group)
别名 CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
计算分子量 742 aa, 82 kDa
GenBank蛋白编号BC004372
基因名称 CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_2934433
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP16070
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD44 antibody CL488-65063Download protocol
Standard Protocols
Click here to view our Standard Protocols