验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
| FC | See 2 publications below |
产品信息
FITC-65063 targets CD44 in IF, FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF, FC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Purified T cells from human lymph nodes 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD44 molecule (Indian blood group) |
| 别名 | Epican, ECMR-III, ECMR III, CSPG8, CDW44 |
| 计算分子量 | 742 aa, 82 kDa |
| GenBank蛋白编号 | BC004372 |
| 基因名称 | CD44 |
| Gene ID (NCBI) | 960 |
| ENSEMBL Gene ID | ENSG00000026508 |
| RRID | AB_2883753 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P16070 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD44 antibody FITC-65063 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation. | ||
Free Radic Biol Med Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization | ||