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Mouse CD45.2 Recombinant antibody, PBS Only

CD45.2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241543F9

Cat no : 84637-1-PBS

Print datasheet

Synonyms

Cd45, Ptprc, Leukocyte common antigen, LCA, B220

验证数据展示

  • WB analysis using 84637-1-RR (same clone as 84637-1-PBS)

    WB analysis using 84637-1-RR (same clone as 84637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84637-1-RR

    Affinity and Kinetic Characterization of 84637-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84637-1-RR against Mouse CD45.2 were performed. The affinity constant is 23.9 nM.

产品信息

84637-1-PBS targets CD45.2 in WB, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Cd45, Ptprc, Leukocyte common antigen, LCA, B220
Observed Molecular Weight200-220 kDa
Gene Symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).