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CD45.2 Recombinant antibody

CD45.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241543F9

Cat no : 84637-1-RR

Print datasheet

Synonyms

Cd45, Ptprc, L-CA, LCA, EC:3.1.3.48

验证数据展示

  • WB analysis using 84637-1-RR

    WB analysis using 84637-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse spleen using 84637-1-RR

    IF Staining of mouse spleen using 84637-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD45.2 antibody (84637-1-RR, Clone: 241543F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 84637-1-RR

    IHC staining of mouse spleen using 84637-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84637-1-RR (CD45.2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84637-1-RR

    Affinity and Kinetic Characterization of 84637-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84637-1-RR against Mouse CD45.2 were performed. The affinity constant is 23.9 nM.

经过测试的应用

Positive WB detected inmouse thymus tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84637-1-RR targets CD45.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Cd45, Ptprc, L-CA, LCA, EC:3.1.3.48
Observed Molecular Weight200-220 kDa
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_3672096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

实验方案

Product Specific Protocols
WB protocol for CD45.2 antibody 84637-1-RRDownload protocol
IHC protocol for CD45.2 antibody 84637-1-RRDownload protocol
IF protocol for CD45.2 antibody 84637-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols