CoraLite®555-conjugated CD45 Monoclonal antibody

CD45 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL555-60287

产品说明书

CloneNo. 4E9B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC, IF-P

4E9B2, B220, EC:3.1.3.48, GP180, L CA

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inmouse spleen tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60287-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-60287 targets CD45 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CD45 fusion protein Ag13712 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 4E9B2, B220, EC:3.1.3.48, GP180, L CA
计算分子量 1304 aa, 147 kDa
观测分子量 220 kDa
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2923894
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08575
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596). 

What is the molecular weight of CD45?

The molecular weight of CD45 is a 180-240 kDa. 

What are the isoforms of CD45?

Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). 

What is the tissue specificity of CD45?

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). 

What are the post-translational modifications of CD45?

CD45 can be heavily N- and O-glycosylated (PMID: 1702721). 

What is CD45's involvement in disease?

Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992).


实验方案

Product Specific Protocols
IF protocol for CL555 CD45 antibody CL555-60287Download protocol
Standard Protocols
Click here to view our Standard Protocols
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