验证数据展示
经过测试的应用
Positive IF/ICC detected in | mouse splenocytes |
Positive FC detected in | mouse splenocytes |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
65139-1-Ig targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.
经测试应用 | IF/ICC, FC Application Description |
文献引用应用 | IF |
经测试反应性 | mouse |
文献引用反应性 | mouse |
免疫原 | Abelson murine leukemia virus-induced pre-B tumor cells 种属同源性预测 |
宿主/亚型 | Rat / IgG2a, kappa |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | protein tyrosine phosphatase, receptor type, C |
别名 | Cd45, Ptprc, RA3-6B2, Leukocyte common antigen, LCA |
GenBank蛋白编号 | BC028512 |
基因名称 | CD45 |
Gene ID (NCBI) | 19264 |
RRID | AB_2918426 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Affinity purification |
UNIPROT ID | P06800 |
储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.
实验方案
Product Specific Protocols | |
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IF protocol for CD45R (B220) antibody 65139-1-Ig | Download protocol |
FC protocol for CD45R (B220) antibody 65139-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |