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PE Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

human, mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

RA3-6B2

Cat no : PE-65139

Print datasheet

Synonyms

CD45R, Cd45, Ptprc, Leukocyte common antigen, LCA

验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using PE-65139

    FC experiment of C57BL/6 mouse splenocytes using PE-65139

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD45R (B220) (PE-65139, Clone: RA3-6B2) or 0.2 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3), and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes, human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65139 targets CD45R (B220) in FC applications and shows reactivity with human, mouse samples.

Tested Applications FC Application Description
Tested Reactivity human, mouse
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45R, Cd45, Ptprc, Leukocyte common antigen, LCA
GenBank Accession NumberBC028512
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_2883921
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06800
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

实验方案

Product Specific Protocols
FC protocol for PE CD45R (B220) antibody PE-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols