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Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HI100

Cat no : 65108-1-Ig

Print datasheet

Synonyms

CD45, B220, HI100, GP180, EC:3.1.3.48

验证数据展示

  • FC experiment of human PBMCs using 65108-1-Ig

    FC experiment of human PBMCs using 65108-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65108-1-Ig

    FC experiment of human PBMCs using 65108-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65108-1-Ig targets CD45RA in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, B220, HI100, GP180, EC:3.1.3.48
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2881472
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08575
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
FC protocol for CD45RA antibody 65108-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols