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APC Anti-Human CD45RA (HI100)

CD45RA Monoclonal Antibody for IF/ICC, IF-P, FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

IF/ICC, IF-P, FC

Conjugate

APC Fluorescent Dye

CloneNo.

HI100

Cat no : APC-65108

Print datasheet

Synonyms

CD45, B220, L CA, HI100, GP180

验证数据展示

  • FC experiment of human PBMCs using APC-65108

    FC experiment of human PBMCs using APC-65108

    1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD45RO and 5 ul APC Anti-Human CD45RA (HI100) (APC-65108, Clone: HI100) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65108

    FC experiment of human PBMCs using APC-65108

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD45RA (HI100) (APC-65108, Clone: HI100) (red) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11) (blue). Cells were not fixed. Lymphocytes were gated.

  • IF Staining of human tonsillitis using APC-65108

    IF Staining of human tonsillitis using APC-65108

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using APC CD45RA antibody (APC-65108, Clone: HI100 ) at dilution of 1:10. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human peripheral blood mononuclear cells using APC-65108

    IF Staining of human peripheral blood mononuclear cells using APC-65108

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using APC Anti-Human CD45RA (APC-65108, Clone: HI100) and CoraLite®488-conjugated Anti-Human CD45RO (CL488-65150, Clone: UCHL1) at dilution of 1:300.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65108 targets CD45RA in IF/ICC, IF-P, FC applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P, FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, B220, L CA, HI100, GP180
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2882997
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08575
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
IF protocol for APC CD45RA antibody APC-65108Download protocol
FC protocol for APC CD45RA antibody APC-65108Download protocol
Standard Protocols
Click here to view our Standard Protocols