APC Anti-Human CD45RA (HI100)
CD45RA Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG2b, kappa
Reactivity
human
Applications
IF/ICC, FC
Conjugate
APC Fluorescent Dye
CloneNo.
HI100
验证数据展示
经过测试的应用
Positive IF/ICC detected in | human peripheral blood mononuclear cells |
Positive FC detected in | human peripheral blood lymphocytes |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
APC-65108 targets CD45RA in IF/ICC, FC applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC Application Description |
Tested Reactivity | human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | protein tyrosine phosphatase, receptor type, C |
Synonyms | CD45, B220, HI100, GP180, EC:3.1.3.48 |
GenBank Accession Number | BC014239 |
Gene Symbol | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
RRID | AB_2882997 |
Conjugate | APC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P08575 |
Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
实验方案
Product Specific Protocols | |
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IF protocol for APC CD45RA antibody APC-65108 | Download protocol |
FC protocol for APC CD45RA antibody APC-65108 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |