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Anti-Human CD45RA (HI100), PBS Only

CD45RA Monoclonal Antibody for Indirect ELISA

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

Indirect ELISA

Conjugate

Unconjugated

CloneNo.

HI100

Cat no : 65108-1-PBS

Print datasheet

Synonyms

CD45, B220, HI100, GP180, EC:3.1.3.48

验证数据展示

  • FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    FC experiment of human PBMCs using 65108-1-Ig (same clone as 65108-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD45RA (65108-1-Ig, Clone:HI100) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65108-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

产品信息

65108-1-PBS targets CD45RA in Indirect ELISA applications and shows reactivity with human samples.

Tested Applications Indirect ELISA Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, B220, HI100, GP180, EC:3.1.3.48
GenBank Accession NumberBC014239
Gene Symbol CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08575
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).