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Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody, PBS Only

CD45RA Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-33

Cat no : 65578-1-PBS

Print datasheet

Synonyms

Lca, Ptprc, CD45, RT7, OX-33

验证数据展示

  • FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

    FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

  • FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

    FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

产品信息

65578-1-PBS targets CD45RA in FC applications and shows reactivity with rat samples.

Tested Applications FC Application Description
Tested Reactivity rat
Immunogen Leukocyte common antigen purified from rat splenocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Lca, Ptprc, CD45, RT7, OX-33
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol CD45
Gene ID (NCBI) 24699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).