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Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody

CD45RA Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-33

Cat No : 65578-1-MR

Print datasheet

Synonyms

CD45, L CA, Lca, Ptprc, RT7, T200

验证数据展示

  • FC experiment of rat splenocytes using 65578-1-MR

    FC experiment of rat splenocytes using 65578-1-MR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

  • FC experiment of rat splenocytes using 65578-1-MR

    FC experiment of rat splenocytes using 65578-1-MR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inrat splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65578-1-MR targets CD45RA in FC applications and shows reactivity with Rat samples.

Tested Applications FC Application Description
Tested Reactivity Rat
Immunogen Leukocyte common antigen purified from rat splenocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, L CA, Lca, Ptprc, RT7, T200
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol Ptprc
Gene ID (NCBI) 24699
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
FC protocol for CD45RA antibody 65578-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols